scReadSim.scRNA_GenerateBAM.SubstiError

scReadSim.scRNA_GenerateBAM.SubstiError(real_error_rate_file, outdirectory, synthetic_fastq_prename)[source]

Generate random errors for single-end sequencing reads according to input real data error rates.

Parameters:

  • real_error_rate_file (str) – Path to software fgbio jar script.

  • outdirectory (str) – Specify the output directory of the synthteic FASTQ file with random errors.

  • synthetic_fastq_prename (str) – Specify the base name of the synthetic erroneous reads’ FASTQ files.